Reymond Research Group

University of Bern


Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers

The research article: Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers just got published in RSC Chemical Communications.

There is currently a lack of efficient reagents to transfect cells with large plasmid DNA, which would be enabling tools for gene editing using CRISPR/Cas9 technology. Herein we report the discovery of peptide dendrimer Z22 as a non-viral vector for transfecting large CRISPR/Cas9 pDNA into 3D-tumor spheroids with exceptionally high efficiency, low cytotoxicity and low immunogenicity.

Author(s): Susanna Joelle Zamolo, Tamis Darbre and Jean-Louis Reymond